CRC/TRR 175/3: Chloroplast Ribonucleoprotein CP29A – adjusting chloroplast RNA pools during cold acclimation responses (SP A02)

Previously, we showed that CP29A creates cold-triggered droplets, depending on its prion-like domain, crucial for splicing, translation, and cold tolerance. Our current focus is to unravel the makeup of CP29A granules, their function in young and proliferating cells, and their role in RNA processing during cold exposure. We will use proximity labeling and CLIP-Seq to determine CP29A granule components, suppressor screens to explore genetic interactions, RNA-Seq and single-cell RNA-Seq on shoot tips to identify cold-induced molecular issues, and investigate RH3, a newly found phase-separating associate of CP29A.