CooC2/AcsF and Cfd1/Nbp35: Maturation of Complex Fe/S-Clusters by MinD-Type ATPases (PP 1927 – Iron/Sulfur for Life)

Anaerobic bacteria and archaea use homologous enzymes to convert CO2 and H2 to acetyl-CoA and methane, which determine since the dawn of life the global carbon cycle on earth. At first we want to investigate whether the homologous enzymes from archaea have the same function as the bacterial CooC2 protein. Another research objective is to search out, how the the high affinity for Ni is generated in the CooC2-apoACS complex and which role ATP plays in the maturation. Furthermore we want to compare the insertion strategy of CooC2 with a different type MinD metal-insertase, Cfd1/Nbp35.